Plasmid-mediated transfer of the blaNDM-1 gene in Gram-negative rods

Detection and Occurrence of Plasmid-Mediated AmpC in Highly Resistant Gram-Negative Rods

OBJECTIVES The aim of this study was to compare the current screening methods and to evaluate confirmation tests for phenotypic plasmidal AmpC (pAmpC) detection. METHODS For this evaluation we used 503 Enterobacteriaceae from 18 Dutch hospitals and 21 isolates previously confirmed to be pAmpC positive. All isolates were divided into three groups: isolates with 1) reduced susceptibility to cef...

Beta-lactamase in gram-negative rods: the relationship between penicillinase and R plasmids in gram-negative rods.

Concomitant with the extensive use of antibiotics, the number of multiple antibiotic-resistant strains has been increasing. Since resistance is mainly mediated by R plasmids, we undertook to investigate the characteristics of R plasmid-determined beta-lactamase in 6 Gram-negative rods. The beta-lactamase produced by each organism was classified by its substrate: type P which attacks penicillins...

Plasmid - mediated horizontal gene transfer is a coevolutionary process 1

Phenotypic identification & molecular detection of blandm-1 gene in multidrug resistant Gram-negative bacilli in a tertiary care centre

BACKGROUND & OBJECTIVES Carbapenemase-producing Enterobacteriaceae isolates have been increasingly identified worldwide. Though molecular data regarding New Delhi metallo-beta-lactamase-1 (NDM-1) producers are available, data regarding their rate of infection in a hospital setting and percentage among different clinical isolates are scarce. Hence, this study was undertaken to determine the occu...

Polyclonal bacteremia due to gram-negative rods.

The frequency of, risk factors for, and outcome of polyclonal gram-negative bacteremia are still unknown. We investigated them in a prospective cohort study of patients for whom a blood culture yielded >/=1 species of gram-negative aerobic rod. For each patient, pulsed field gel electrophoresis (PFGE) was performed on 4 colonies of each morphologic type. Episodes of bacteremia were considered p...